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Laboratory Recepies...... M Sc Biochemistry Laboratory Schedule: M. Sc. Biochemistry (III semester) BC-604 Practical Schedule (2005-2006)
M. Sc. Microbiology (III semester) Mi-604 Enzymology (2005-2006) List of Practicals 1. Estimation of Reducing sugar by DNS method 2. Estimation of Pi by Fiske and Subarrow Method 3. Invertase Assay 4. Acid-Alkaline phosphatase assays 5. Progress curve 6. Enzyme curve 7. Substrate saturation curve 8. Optimization of pH for invertase activity 9. Analysis of Substrate saturation data by various plots 10. Inhibition kinetics 11. Thermal kinetics 12. Optimization of Temperature for invertase activity 13. Effect of temperature on Rate of reaction and determination of activation energy 14. Effect of temperature on enzyme stability 15. A visit to Protein Data Bank and ExPASy 16. Study of Protein structures by Rasmol, Protein Explorer, Deepview. 17. Pairwise and multiple sequence alignment 18. Structural alignment 19. Superimposition of 2 protein structures for comparision 20. Generation of phylogenetic tree using PHYLIP, treeview. | |||||||||||||||||||||||||