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Laboratory Recepies......

M Sc Biochemistry Laboratory Schedule:

Practicals Related to BC 604


M Sc Microbiology Laboratory Schedule:

Practicals Related to Mi 604










M. Sc. Biochemistry (III semester) BC-604 Practical Schedule (2005-2006) 

No

Period (From to)

Practicals

1

18-23rd July

1.        Setting up of Winogradsky’s column for studying nitrogen and sulphur metabolizing bacteria. (18th July) 

2.        Setting up of Winogradsky’s column for enrichment of phenol degrading bacteria. 

3.        Isolation of pure cultures by streak plate and spread method. 

4.        To determine correlation between viable count and turbidity of bacterial culture. 

2

15-20th August

5.        Growth curve of bacteria by turbidimetric and viable count technique 

6.        Determination of generation time of bacteria 

7.        Isolation of organic acid producing bacteria 

8.        Screening of antibiotic producing bacteria by crowded plate technique 

3

12-17th September

9.        Determining b-galactosidase activity from whole cells of E. coli 

10.     Isolation of rhizobia, azotobacter, yeast, actinomycetes from soil. 

4

10-15th October

11.     Isolation and identification of Gram-positive bacilli using biochemical characteristics 

5

7-12th November

12.     Bioassay of penicillin/streptomycin 

13.     Chemical estimation of penicillin/ streptomycin. 

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M. Sc. Microbiology (III semester) Mi-604 Enzymology (2005-2006)


List of Practicals

1. Estimation of Reducing sugar by DNS method

2. Estimation of Pi by Fiske and Subarrow Method

3. Invertase Assay

4. Acid-Alkaline phosphatase assays

5. Progress curve

6. Enzyme curve

7. Substrate saturation curve

8. Optimization of pH for invertase activity

9. Analysis of Substrate saturation data by various plots

10.    Inhibition kinetics

11.    Thermal kinetics

12.    Optimization of Temperature for invertase activity

13.    Effect of temperature on Rate of reaction and determination of activation energy

14.    Effect of temperature on enzyme stability

15.    A visit to Protein Data Bank and ExPASy

16.    Study of Protein structures by Rasmol, Protein Explorer, Deepview.

17.    Pairwise and multiple sequence alignment

18.    Structural alignment

19.    Superimposition of 2 protein structures for comparision

20.    Generation of phylogenetic tree using PHYLIP, treeview.

 

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Last Updated 13th July 2005