Department of Biosciences

M Sc II Semester Microbiology / Biotechnology

Mi 503 / BT 503 Internal Examination

 

Date : 19th February 2004

Max Marks : 30

 

 

Q. 1     Attempt: ( ANY THREE )                                                                               (15)

 

a)      Draw and explain ‘Rotary vacuum filters’.

b)      Explain transesterification of palm oil into biodiesel.

c)      Explain temperature/pH control loop in general and in our NBS fermenter in particular.

d)      Explain with one example the use of R DNA technology in strain improvement.

e)      Write a note on ‘Lyophilization’.

f)        Draw and explain ‘pinch valve’.

g)      List methods of growth measurement and explain turbidometry in detail.

h)      Discuss advantages of fermented foods.

i)        Draw and explain the design of ‘Deep shaft reactor’.

 

Q. 2     Compare batch and continuous sterilization. Draw a neat diagram of continuous sterilization process.                                                                                          (05)

                                                                        OR

Q. 2     Discuss ‘scale up’ with respect to batch sterilization.                              (05)

 

 

Q. 3     Draw immobilized enzyme reactors and explain any one.                                    (05)

 

 

Q. 4     What is KLa ? Explain sulfite oxidation method for its determination.      (05)

                                                                        OR

Q. 4     What is KLa ? Explain how it is affected by medium rheology.                (05)

 

 

 

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Sardar Patel University

Department of Biosciences

M Sc II Semester Microbiology

Mi 503 Bioprocess and Fermentation Technology

External Theory Examination

Date : 3rd December 2004

Time: 2:30 pm to 5:30 pm

Max Marks : 70

 

Q. 1     Explain the Design of:                                                                            (12)

a)      Pressure cycle reactor

b)      Portable laboratory fermenter

OR

Q. 1     Explain with the help of neat diagram the design of a typical mechanically driven fermenter.                                                                                                         (12)

 

Q. 2     Explain with a neat diagram batch and continuous sterilization.                (12)

OR

Q. 2     Explain filter designing in Air sterilization.                                                           (12)

 

Q. 3     What is KLa? Discuss methods used to determine KLa.                         (10)

OR

Q. 3     Explain the design of equipment used for aseptic inoculation on large scale.(10)

 

Q. 4     Write an essay on “Raw Materials”.                                                                  (12)

OR

Q. 4     Discuss the microprocessor based control for the fermentation processes.          (12)

 

Q. 5     Write a note on: (any four)                                                                               (24)

 

a)      Temperature measurement

b)      Methods of cell separation

c)      Biosensors

d)      Enzyme reactors

e)      Inoculum development program.

f)        Fermentation economics

g)      Plug flow reactors

h)      Growth kinetics

 

 

 

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