M.Sc (I semester) Microbiology. Internal Examination

Paper: 402- Biophysics and Instrumentation.

9th September,2003.                                                                                              30 marks

   2.00 – 3.00 pm

 

 

I.            Attempt any two:                                                                                   (5 marks)

a)                  Draw a labeled diagram of a dipeptide

 

b)                 Draw a titration curve of 0.1 M Glycine

 

c)                  “No single method is sufficient to establish the homogeneity of purified protein” Justify.

 

II.         “ The phase-contrast microscopy is a special adaptation of the light microscopy which helps in obtaining clear images of living or unstained cells”. Explain.

                                                                                                                           (5 marks)

 

III.            Attempt any three:                                                                             (15 marks)

 

a)      What are zonal rotors? Explain the working and applications of an Elutriator rotor.

 

b)      “The medium as well as the sample influence the rate sedimentation during centrifugation”- Justify with suitable examples.

 

c)      Enumerate the factors affecting separation in electrophoresis. Discuss the role of buffer in determining electrophoretic mobility.

 

d)      Describe capillary electrophoresis. What are the advantages of this technique?

 

IV.       Name the appropriate rotor type against the application:                        (2 marks)

a)                  Continuous flow –

b)                 Differential centrifugation-

c)                  Isopycnic centrifugation –

d)                 Rate zonal centrifugation –

 

V.        Write short notes on any one:                                                               (3 marks)

 

a)                  Properties of density gradient materials

 

b)                 Paper chromatography.

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M.Sc (I semester) Biotechnology. Internal Examination

Paper: 402- Biophysics and Instrumentation.

9th September,2003.                                                                                              30 marks

   2.00 – 3.00 pm

 

 

I.            Attempt any two:                                                                                   (5 marks)

a)      Draw a labeled diagram of a dipeptide

 

b)      Draw a titration curve of 0.1 M Glycine

 

c)      “No single method is sufficient to establish the homogeneity of purified protein” Justify.

 

II.         “ The phase-contrast microscopy is a special adaptation of the light microscopy which helps in obtaining clear images of living or unstained cells”. Explain.

                                                                                                                           (5 marks)

 

III.            Attempt any three:                                                                             (15 marks)

 

a)      What are zonal rotors? Explain the working and applications of an Elutriator rotor.

 

b)      “The medium as well as the sample influence the rate sedimentation during centrifugation”- Justify with suitable examples.

 

c)      Enumerate the factors affecting separation in electrophoresis. Discuss the role of buffer in determining electrophoretic mobility.

 

d)      Describe capillary electrophoresis. What are the advantages of this technique?

 

IV.       Name the appropriate rotor type against the application:                        (2 marks)

a)      Continuous flow –

b)      Differential centrifugation-

c)      Isopycnic centrifugation –

d)      Rate zonal centrifugation –

 

V.        Write short notes on any one:                                                               (3 marks)

 

a)      Properties of density gradient materials

 

b)      Paper chromatography.

xxxxxxxxxx

 

 

 

M.Sc (I semester) Biochemistry Internal Examination

Paper: 402- Biophysics and Instrumentation.

9th September,2003.                                                                                              30 marks

   2.00 – 3.00 pm

 

I           (a) Explain the importance of dissociation constants of weak acids in the formation and function of a buffer.

            (b) Distinguish between: Standard free energy change and Free energy change.                                                                                                                 (5 marks)

 

II.         “ The phase-contrast microscopy is a special adaptation of the light microscopy which helps in obtaining clear mages of living or unstained cells”. Explain.

                                                                                                                           (5 marks)

 

III.            Attempt any three:                                                                             (15 marks)

 

a)      What are zonal rotors? Explain the working and applications of an Elutriator rotor.

 

b)      “The medium as well as the sample influence the rate sedimentation during centrifugation”- Justify with suitable examples.

 

c)      Enumerate the factors affecting separation in electrophoresis. Discuss the role of buffer in determining electrophoretic mobility.

 

d)      Describe capillary electrophoresis. What are the advantages of this technique?

 

IV.       Name the appropriate rotor type against the application:                        (2 marks)

a)      Continuous flow –

b)      Differential centrifugation-

c)      Isopycnic centrifugation –

d)      Rate zonal centrifugation –

 

V.        Write short notes on any one:                                                               (3 marks)

 

a)      Properties of density gradient materials

 

b)      Paper chromatography.

 

c)      Role of weak interactions in the maintenance of macromolecules’ structure in a cell.

xxxxxxxxxx

 

 

 

 

 

 

 

M.Sc (III semester) Botany. Internal Examination

Paper: 603- Medical Botany & Phytochemistry

9th September,2003.                                                                                              30 marks

11 am – 12 noon

 

 

I.            Attempt any four:                                                                                  (4x6 = 24)

 

a)      What is the role of Ethanobotany in drug discovery? Discuss the merits and

demerits of ethanobotanical data in drug development with suitable examples.

 

b)      What are glycosides? Name the sources of anthraquinone glycosides. Outline

the cultivation, chemical constituents and uses of Senna.

 

c)      Define Biotransformation. Explain the different methods employed for

Biotransformation.

 

d)      Outline the method for the extraction of Opium alakaloids with a brief note

on the medical uses of these alkaloids.

 

e)      Describe the chemical constituents of Digitalis. Briefly discuss the various

chemical tests used to check the authenticity of the drug.

 

f)        Give an account of the in vivo and in vitro production and uses of Ergot

alkaloids.

 

 

II.         Write short notes on any two:                                                                  (2x3 = 6)

 

a)                  Steam distillation of essential oils

b)                 Preparation of Cocaine

c)                  Uses of Belladonna