P.G.Department of Biosciences

Sardar Patel University, Vallabh Vidyanagar

IIIrd Semester Biochemistry Theory Test in Microbial Biochemistry & Applied Microbiology

Date: 11/09/2003                    Max marks: 30                    Time: 11.00 a.m. to 12.00 noon

 

Q.1 Describe general methods for classifying bacteria.                                                           (07)

 

Q.2 Describe methods for preservation of industrially important microorganisms.                        (05)

                                                           

Q.3 Write a note on ‘Synchronous cultures’.                                                                      (04)

 

Q.4 Answer the following questions in brief:                                                                 (10)

  1. Describe advantages of continuous enrichment culture over batch enrichment culture.
  2. What is the composition of nutrient broth?
  3. How can you demonstrate that a microbe possesses antibiotic activity or not?
  4. 100 cells of E. coli (generation time of 30 min) were grown for 5 hours at 350C. How many cells would be there after 5 hours.
  5. Distinguish between chemostat and turbidostat.

 

Q.5 Choose the right answers from the given options for the following statements respectively: (04)

 

1. In prokaryotic cells, ETC is carried out in

(a)   Inner mitochondrial membrane

(b)   Outer mitochondrial membrane

(c)   Endoplasmic reticulum

(d)   Plasma membrane.

2. The best carbon source for a given microorganism’s growth is the one with

(a)    high Ks value

(b)    low Ks value

(c)    high umax

(d)    low umax

3. Mutant of Corynebacterium glutamicum that produces high amount of glutamate at low concentration of biotin is because

(a)    biotin inhibits glutamate production

(b)    biotin inhibits Acetyl CoA carboxylase

(c)    lack of biotin develops deficient phospholipid in plasma membrane

(d)    biotin increases the Yp/x for glutamate.

4. A type strain is the one

(a)    Which is most typical of all strains included in the species

(b)    Which is not important to maintain because it is going to degenerate anyway

(c)    Which is the permanent reference specimen for the species

(d)    Which is isolated first of all strains.

 

 

 

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P.G.Department of Biosciences

Sardar Patel University, Vallabh Vidyanagar

IIIrd Semester Biochemistry Practical Test in Microbial Biochemistry & Applied Microbiology

Date: 12/09/2003                    Max marks: 25                                Time:

 

Q.1 Estimate the number of E.coli cells in a given suspension, which is prepared from the culture, inoculated with 10 cells and allowed to grow for 2 hrs.                                                                       (15)

OR

Q.1 Classify microorganisms based on their temperature requirement. Determine the optimum temperature for the given microorganism.                                                                          (15)

 

Q.2 Prepare enrichment culture and isolate the bacteria from the given soil sample which can utilize____________________ selectively as a source of carbon.                                                 (10)

 

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P.G.Department of Biosciences

Sardar Patel University, Vallabh Vidyanagar

IIIrd Semester Biochemistry Practical Test in Microbial Biochemistry & Applied Microbiology

Date: 12/09/2003                    Max marks: 25                                Time:

 

Q.1 Estimate the number of E.coli cells in a given suspension, which is prepared from the culture, inoculated with 10 cells and allowed to grow for 2 hrs.                                                                       (15)

OR

Q.1 Classify microorganisms based on their temperature requirement. Determine the optimum temperature for the given microorganism.                                                                          (15)

 

Q.2 Prepare enrichment culture and isolate the bacteria from the given soil sample which can utilize____________________ selectively as a source of carbon.                                                 (10)

 

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DEPARTMENT OF BIOSCIENCES

SARDAR PATEL UNIVERSITY

M.Sc. (Biochemistry) IInd Semester Practical Test

Lab II - Biochemical and Environmental Toxicology

Date: 29th March 2003                                                    Time: 10.30 a.m. onwards         

Q.1 Find out the concentration of heavy metal that is inhibitory to the growth of given pathogenic fungus. Submit your petriplates along with the results.              (20)

OR

Q.1 Find out the lethal and sublethal time using undiluted and 1:1 diluted concentration of given toxin. Also find out its LD50 at undiluted and 1:1 diluted concentrations.                                                                                     (20)

OR

Q.1 Estimate the proteinase inhibitory activity of the given toxin.                   (20)

OR

Q.1 Find out the chloride content of given effluent sample. Comment on your results.(20)

 

Q.2 Using the given data draw the graph of log concentration of Carbaryl Vs. percent mortality and find out LC50 of Carbaryl.                                                          (05)

 

Conc. of carbaryl (in ppm)                Number of fish exposed       Number of fish died

 

            4                                                          12                                            0

            6                                                          12                                            0

            8                                                          12                                            4

            10                                                        12                                            10

            12                                                        12                                            12

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SARDAR PATEL UNIVERSITY

DEPARTMENT OF BIOSCIENCES

M.Sc. II Semester Examination                                                      Date: 27/04/2004

BC-502 Biochemical and Environmental Toxicology           Time: 3.00 to 6.00 p.m.

                                                                                                               Max. marks : 70

Note:            Numbers in parenthesis indicate marks

            Write two sections in separate answer books

 

SECTION I

 

Q.1      (a) Describe phase I and phase II reactions of biotransformation of xenobiotics with

      appropriate examples.                                                                                            (08)

            (b) Explain:             (i) Synergism,                    ii) Antagonism,            iii) Toxicokinetics            and                               (iv) Therapeutic Index                                                   (08)

 

OR

 

Q.1      (a) Describe chronic health effects of air pollution.                                            (06) (b) Enumerate arsenic and lead poisoning effects. Name their antidotes.        (05)        

(c) Define Toxicology. Write on general mechanism of toxicity.                       (05)      

 

Q.2            Attempt any three of the followings:                                                                   (12)

 

(a)    Scope of toxicology

(b)   Dose-response relationship

(c)    Tissue specificity of toxicity

(d)   Factors influencing toxicity

(e)    Structure activity relationship

(f)     Therapeutic drug monitoring                        

 

 

SECTION II

 

Q.3      (a) Explain the following in brief:                                                                (08)

 

(i)                 First pass metabolism effect

(ii)                General and specific measures for management of poisoned patients

(iii)              Teratogenesis

(iv)              Potentiation

 

 (b) Describe ‘Biochemistry of Carcinogenesis’. Which test is performed to access carcinogenicity of a substance? How?                                            (07)

 

OR

Q.3      (a) Explain the toxicity of following toxicants:                                            (10)

i)                    Polychlorinated biphenyls (PCB)

ii)                  DDT and other organochlorine insecticides

 

(b) Explain various layers of atmosphere.                                               (05)

 

Q.4      (a) Answer the following in brief:                                                   (10)

 

i)                    If LD50 of Parathion is 100 times less than Malathion, which is more toxic?

ii)                  Enumerate the epidemics occurred due to mercury poisoning.

iii)                 Explain the possible ways in which sulfur dioxide may react in the atmosphere.

iv)                What can be given in toxicity due to Paracetamol overdosage?

v)                  What is Cholestasis?

 

(b) Differentiate between:                                                                                (05)

i)                    Agonist and Antagonist

ii)                  Pharmacodynamics and Pharmakinetics

 

OR

 

Q.4      Write an explanatory note on the following:                                                        (15)

i)                    Green house gases and global warming

ii)                  Biopesticides

iii)                 Herbicides

 

Q.5      Explain any three of the followings:                                                          (12)

i)                    Acid rain

ii)                  Resistance against pests/microbial pathogens in plants using defense pathways

iii)                 Ozone layer destruction

iv)                Fate of atmospheric CO

v)                  Metabolism of Paracetamol

vi)                Use of Drosophila in toxicity testing